Innovative Cell Technologies

Innovative Cell Technologies (ICT), a San Diego based company specialises in the production and supply of Accutase™; a solution for the detachment of adherent cells, and Accumax™; a cell aggregation dissociation solution for accurate cell counting.

Accutase™: a solution for the detachment of adherent cells

Accutase™ was developed to meet the most demanding needs for gentle and effective detachment of adherent cells. In the last two years, it has demonstrated outstanding performance with hESC cells. Download a scientific paper demonstrating Accutase's ability to grown hESC cells in a monolayer.
Accutase™ has also been proven to be effective in detaching:fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, stellates, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080fibrosarcoma cells, and Sf9 insect cells. Download a list of recent publications using Accutase.
Unlike Trypsin, Accutase™ does not need to be washed out or neutralized; the addition of more media inactivates Accutase™, thus saving time in cell passaging work. No more washes!!
Accutase™ gently and rapidly lifts off adherent cells without destroying epitopes being measured with flow cytometry.
It combines protease and collagenolytic activities which maximizes its versatility for cell detachment.
Accutase™ does not contain mammalian or bacterial-derived products.
Accutase™ is available in a ready-to-use formulation, quality controlled for in vitro cell culture applications.

Accutase™
Accutase Chart
CELL DETACHMENT: Human MG63 fibrosarcoma cells cultured on tissue culture treated dishes in DMEM + 10% FBS were treated with ACCUTASE. Treatment results in rapid cell detachment, a single cell suspension, and high viability. ACCUTASE is gentle on cells; viability was 97 ± 3% even after 45 minutes in ACCUTASE.

Accumax™ Accumax Chart
BHK cells, and a hybridoma were grown in suspension in serum-free or protein-free medium. Representative cell aliquots were treated with an equal volume of PBS or ACCUMAX and incubated for 5 minutes at 37C. Cell number was then determined with a Coulter Counter.

Accumax™: a cell aggregation dissociation solution for accurate cell counting

Accumax™ has been developed to meet the most demanding needs for an effective cell aggregate dissociation solution.

It has been proven to be effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, and others.

Accumax™ gently and rapidly dissociates cell clumps to yield single cell suspensions for accurate and reproducible cell counts.

Combining protease, collagenolytic, and DNase activities maximizes its versatility for cell aggregate dissociation.

Accumax™ does not contain mammalian or bacterial-derived products.

Accumax™ is available as a ready-to-use liquid, quality controlled for in vitro cell culture applications.

Download the Accutase datasheet here.

Download the Accumax datasheet here.

Accutase Bottle

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