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Arteriocyte:
NANEX™ Cell Culture and Expansion System

Arteriocyte, is a leading clinical stage biotechnology company dedicated to developing novel stem cell research products and medical devices for unmet research and clinical needs.

Arteriocyte has developed the NANEX™ Cell Expansion System as an enabling technology for expansion of different types of primary cells for basic research, therapeutics, drug discovery and screening. With NANEX™ technology, Arteriocyte initiated the first non-cancer clinical trial in the US using allogenic HSCs for the treatment of Critical Limb Ischemia patients. Further disease targets are being validated.

Innovative Solution for Hematopoietic Stem Cells/Progenitor Cells (HSCs/HPCs) Expansion

The NANEX™ Stem Cell Expansion System is designed for the efficient ex vivo expansion of hematopoietic stem cells (HSCs) and progenitor cells (HPCs) derived from human bone marrow and umbilical cord blood. The NANEX™ cell culture substrate is a biofunctional nanofiber-based 3D scaffold that partially mimics the bone marrow stem cell niche. When used in combination with serum-free medium and a cocktail of growth factors, NANEX™ scaffold can facilitate the proliferation of HSCs/HPCs without significant loss of the stem cell phenotype. NANEX™ HSC Expansion Kit is the first and only product on the market that can expand HSCs and HPCs with superior performance and consistency.

Features & Benefits

• Creating in vivo-like culture environment by mimicking bone marrow niche
• Maintain high HSCs/HPCs purity - at least 5 times higher than tissue treated cul-ture plates and poly-amine surfaces on the market
• Significantly expand HSCs/HPCs - yield at least 3 times more CD34+ cells over other expansion products on the market
• Each NANEX kit can yield over 10 million CD34+ cells from less than 100,000 cells, eliminating the donor variability issue for large scale studies and making higher doses possible, greatly assisting translational research
• Easy handling and cell harvesting process
• Expanded cells maintain multipotency and colony forming potential

Product Information

The NANEX™ Hematopoietic Stem Cell Expansion Kit is designed specifically for the ex vivo expansion of HSCs. The kit is optimized and designed to be able to harvest over 10 million HSCs (CD34+ cells) through a 10-day serum free culture (less than 100,000 cells). This not only provides significant cost saving for your lab, but also makes it possible to use the cells from a single donor for multiple experiments, assisting both of the research in basic sciences as well as translational research.

Each NANEX™ HSC Expansion Kit contains everything needed for the efficient HSC expansion:

• NANEX™-Coated 6-well Culture Plates (5 each)
• HSC Expansion Serum Free Media (200 ml)
• HSC Expansion Cytokine Cocktail (1 vial)
• User Manual

NANEX™ Technology Overview

With a biofunctional nanofiber-based surface, NANEX™ is designed to partially mimic the bone marrow environment. In order to expand stem cells the biofunctional surface, together with the surface topography structure, facilitates and promotes rapid in vivo-like cell growth and expansion.

1. NANEX™ fibres under scanning electron microscope (SEM). Typically the fibre diameter ranges from 300nm to 800nm.
2. Structure of bone marrow matrix.
3. 5000X and (d) 10,000X SEM images of Hematopoietic Stem Cells (HSCs) growing on top of the NANEX™ surface.

Experimental and clinical procedures involving stem cells are challenging because of the sparse population of stem cells available in adult tissues. In addition, it has been well documented that cells cultured on 2D culture surface does not mimic the in vivo growth. Arteriocyte's NANEX™ technology is developed to address such challenges.

References

1. Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells. Biomaterials, 27(36): 6043-6051
2. Ex Vivo Nanofiber Expansion and Genetic Modification of Human Cord Blood-Derived Progenitor/Stem Cells Enhances Vasculogenesis. Cell Transplant, 18(3): 305-318
3. Stem Cell Therapy with Overexpressed VEGF and PDGF Genes Improves Cardiac Function in a Rat Infarct Model. PLoS One, 4(10): e7325
4. Hematopoietic Stem Cells: Ex Vivo Expansion and Therapeutic Potential for Myocardial Ischemia. Stem Cells and Cloning: Advances and Applications, 2010(3): 57-68
5. Expansion of Hematopoietic Stem Cells. Genetic Engineering News, 30 (16): 50 - 5
6. Expansion and Characterization of Cord Blood-Derived CD34+ Cells on NANEX™ 3D Nanofiber Scaffold. The International Society of Stem Cell Research 9th Annual Meeting, 2011