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Human Angiogenesis Assay Kit

Catalogue Number ZHA1000

Description

Twenty-four well tissue culture plate containing proliferating early passage normal human endothelial cells co-cultured with early passage normal human interstitial cells in a specially formulated culture medium. These cultures spontaneously develop a network of capillary-like tubules after 10-14 days in culture. Tubule development is believed to closely mimic in vivo angiogenesis and is enhanced by known stimulators of angiogenesis and suppressed by known angiogenesis inhibitors, both in a dose-dependent manner.

The kit contains sufficient culture medium to support development of the assay over the required 14 day period.

In summary:

• Co-culture of primary human endothelial cells with primary human interstitial cells
• No other additions or manipulations except addition of test substances and medium changes
• Kits are shipped at ambient temperature ready to use
• Anastomosing tubule network forms within 10-14 days
• Tubules stained with antibodies to von Willebrand factor (Fig 1c), PECAM-1 or collagen type IV (1a)
• Permits evaluation of both inhibitors and stimulators of angiogenesis
• 24 well format - compatible with replicate samples or secondary screening needs
• Several approaches to analysis of results are available that include tubule length, branch points, tubule area in parallel to angiogenic inhibitor & stimulator specific immunoassays

Perfectly designed for high content screeing studies for:

• Pharmaceutical & Biotechnology companies
  - Secondary screening
  - Preclinical research
  - High Content Screening
  • vascular research
  • cardiovascular research
  • regenerative medicine
  • rheumatoid arthritis
  • Diabetic retinopathy
  • cancer/ tumour regulation
  • wound healing
  • tissue neovascularization
  • embryogenesis
• Cancer Research Institutes
  • tumour regulation
  • non malignant growths
  • metastasis research
• Regenerative Medicine Institutes
  • tissue repair mechanisms
  • tissue engineering
  • tissue neovascularization
  • skin graft research
  • organ & bone re-generation
• Cosmetic Research
  • epidermal regeneration research
  • tissue repair mechanisms/wound healing

Full descriptions of the kit can be found through the following links:

• Angiogenesis overview (.pdf, 1.5mb)
• Angio presentation (.pdf, 7mb)
• AngioKit Product Insert (.pdf, 85kb)
• AngioKit Product